Studying grazing behavior of Cafeteria roenbergensis with two-photon microscopy

Faisal Abedin, University of Texas at El Paso

Abstract

Major innovations in recent years have largely revolutionized the fluorescence imaging. Two-photon fluorescence microscopy is one of them. Two photon fluorescence microscopy has evolved as an alternative to conventional single-photon confocal microscopy and has been shown to provide several advantages. These include three-dimensionally resolved fluorescence imaging of living cells deep within thick, strongly scattering samples, and reduced phototoxicity, enabling long term imaging of photosensitive biological specimens. The inherent three-dimensional resolution of TPE microscopy has been exploited in a number of studies wherein spatial discrimination of fluorescence signals at the micrometer and submicrometer scale within thick biological specimens proved critical. In this study, we used our two-photon microscope to observe grazing behavior of fast moving marine organism Cafeteria roenbergensis with the interaction of bacteria.

Subject Area

Physics|Optics

Recommended Citation

Abedin, Faisal, "Studying grazing behavior of Cafeteria roenbergensis with two-photon microscopy" (2016). ETD Collection for University of Texas, El Paso. AAI10151201.
http://digitalcommons.utep.edu/dissertations/AAI10151201

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