Novel copper (II) mediated transfection nano-systems and biological properties of pyridinium surfactants
Efficacy of gene therapy is relied upon the improvement of gene delivery vectors into mammalian cells. Although viral-based polynucleotide carriers are still considered as the most common method for gene delivery but the cost effectiveness, nonspecific lethal immune response has evoked the renaissance of non-viral synthetic delivery systems. An initial discovery by Felgnar et al showed Cationic Liposomes (CLs) (having an overall positive charge) when mixed with DNA to form CL-DNA complexes can enhance the transfer of polynucleotides into cells followed by its expression. Presently, a flurry of experimental works is taking place for the better understanding of structural characterization and functional efficacy of supramolecular structures (CLs-DNA complex). This effort has contributed CLs delivery system as the most popular rapidly emerging non-viral method for delivering genes in cells as well as for therapeutic clinical trails.^ The stability of supramolecular self-assembled structures relies upon pre-organization of molecular receptors effecting molecular recognition and appropriate manipulation of intermolecular non-covalent interaction, hydrogen bonding arrays and ion coordination sites of participating molecular components. Campa et al first reported a novel class of metal mediated amphiphillic molecules that have the ability to generate discrete supramolecular self-assembly in water and condense into CLs-DNA structures capable of transfecting polynucleotides to mammalian cells via redox and ligand exchanged reactions. Here in this work we report a different class of Cu mediated co-ordination amphiphiles which are designed to self organize via co-ordination and hydrogen bonding interactions to cationic metallo liposomes. These CLs are capable of delivering a gene (pEGFPN1) encodes for green fluorescent protein into mammalian cells (HEK 293 T). ^ We have used transmission electron microscopy (TEM), dynamic light scattering (DLS), and confocal optical microscopy to characterize the structure of CLs-DNA complex and lipoplexes. The transfection efficiency was measured with fluorescent microscopy and fluorescence activated cell sorting (FACS).^
Biology, Cell|Chemistry, Molecular
Pal, Sarit, "Novel copper (II) mediated transfection nano-systems and biological properties of pyridinium surfactants" (2013). ETD Collection for University of Texas, El Paso. AAI1546338.