Studying cellular and molecular interaction with two-photon microscopy

Arifur Rahaman, University of Texas at El Paso

Abstract

Advances in microscopy and fluorescent probes provide new insight into studying cellular and molecular interactions. Two-photon fluorescence microscopy is one of the most important recent inventions in cellular and molecular study. This technology enables noninvasive study at cellular and molecular levels in three dimensions with submicrometer resolution. Two-photon excitation of fluorophores results from the simultaneous absorption of two photons. This excitation process has a number of unique advantages, such as reduced specimen photodamage and enhanced penetration depth. In this study, we used our two-photon microscope to observe predatorial behavior of fast moving bacterivorous marine organism Cafeteria roenbergensis and quantify Mycobacterium phagosomal rupture in macrophages.^

Subject Area

Optics|Biophysics

Recommended Citation

Rahaman, Arifur, "Studying cellular and molecular interaction with two-photon microscopy" (2015). ETD Collection for University of Texas, El Paso. AAI1600343.
http://digitalcommons.utep.edu/dissertations/AAI1600343

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