Pyrene alters serum chemistry, gene expression, and liver lipids in CD-1 mice
Pyrene is a polycyclic aromatic hydrocarbon that is ubiquitous in the environment as a product of the incomplete combustion of fossil fuels. A large amount of literature exists for the toxicity and carcinogenicity of PAHs, but data for pyrene is very limited. In order to further understand the toxic effects of pyrene at nonlethal doses, CD-1 adult male mice were fed pyrene in honey for 7 days. Six mice were assigned to a control group, 5 mice were assigned to the 3 mg/kg pyrene group, and 5 mice were in the 30 mg/kg pyrene group. The differential expression of the CYP1A2, AhR, and ARNT genes, which have been shown to be differentially expressed as a result PAH exposure, was determined by real-time PCR. To determine whether liver damage or fatty acid class alteration occurred, total levels of serum triglyceride, cholesterol and bilirubin were measured. Lipids were extracted from the livers of control and treated mice to determine if fatty acid class alteration occurred using thin layer chromatography. The liver cDNA was also analyzed by microarray and a list of differentially expressed genes was generated. (Abstract shortened by UMI.)^
Health Sciences, Toxicology|Biology, Animal Physiology
Hernandez, Laura Yanira, "Pyrene alters serum chemistry, gene expression, and liver lipids in CD-1 mice" (2004). ETD Collection for University of Texas, El Paso. AAIEP10786.