Cu(II) Lipoplexes Of 1,4,7-Triazacyclononane And Their Biological Properties
In order to aid in the design of future oligonucleotide transport vehicles we synthesized an array of surfactants and test their differences in transfection efficiency. A novel class of surfactant-copper (II) complexes, Cu(TACN-C8)2, Cu(TACN-C10)2, Cu(TACN-C12)2, Cu(TACN-C14)2, Cu(TACN-C16)2, and (TACN-C18)2, were synthesized and fully characterized to generate, through self-assembly, cationic nanoparticles. Supramolecular characterization of nanoparticles and nanopartilce/DNA condensates was conducted through CMC, DLS and microscopy. An evaluation of the transfection efficiency of each surfactant was assessed by transformation of a Hela cell line, for which the highest observed efficiency was 38% corresponding to the surfactant with the dodecyl alkyl chain.