Study of Blinking Statistics in Silver Coated Cobalt Ferrite Single Nanoprticle Using Two-Photon Fluorescence Microscopy

Rajen Kumar Goutam, University of Texas at El Paso

Abstract

Two-photon fluorescence microscopy is a powerful tool to study the molecular and cellular interactions. This technology is a non-invasive approach with the advantage of three-dimensional imaging up to submicron resolution. Two-photon excitation process is the result of simultaneous absorption of two photons that has special features of reduced photodamaged and elongated penetration depth on samples. In this work, blinking statistics of silver-coated Cobalt ferrite (CoFe2O4) single nanocrystal is studied using two-photon fluorescence microscopy. By defining the intensity threshold, the observed fluorescence is divided into two distinct stages: OFF and ON states. Both of these states followed the inverse power law. This power law is different from the well-known three-level quantum jump for blinking.

Subject Area

Physics|Optics|Nanoscience

Recommended Citation

Goutam, Rajen Kumar, "Study of Blinking Statistics in Silver Coated Cobalt Ferrite Single Nanoprticle Using Two-Photon Fluorescence Microscopy" (2019). ETD Collection for University of Texas, El Paso. AAI22587254.
https://digitalcommons.utep.edu/dissertations/AAI22587254

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