Date of Award
2015-01-01
Degree Name
Master of Science
Department
Physics
Advisor(s)
Chunqiang Li
Abstract
Advances in microscopy and fluorescent probes provide new insight into studying cellular and molecular interactions. Two-photon fluorescence microscopy is one of the most important recent inventions in cellular and molecular study. This technology enables noninvasive study at cellular and molecular levels in three dimensions with submicrometer resolution. Two-photon excitation of fluorophores results from the simultaneous absorption of two photons. This excitation process has a number of unique advantages, such as reduced specimen photodamage and enhanced penetration depth. In this study, we used our two-photon microscope to observe predatorial behavior of fast moving bacterivorous marine organism Cafeteria roenbergensis and quantify Mycobacterium phagosomal rupture in macrophages.
Language
en
Provenance
Received from ProQuest
Copyright Date
2015
File Size
61 pages
File Format
application/pdf
Rights Holder
Arifur Rahaman
Recommended Citation
Rahaman, Arifur, "Studying Cellular And Molecular Interaction With Two-Photon Fluorescence Microscopy" (2015). Open Access Theses & Dissertations. 1129.
https://scholarworks.utep.edu/open_etd/1129